Fast Protein Liquid Chromatography (FPLC)

Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the "mobile phase") and a porous solid (the stationary phase). In FPLC the mobile phase is an aqueous solution, or "buffer". The buffer flow rate is controlled by a positive-displacement pump and is normally kept constant, while the composition of the buffer can be varied by drawing fluids in different proportions from two or more external reservoirs. The stationary phase is a resin composed of beads, usually of cross-linked agarose, packed into a cylindrical glass or plastic column

NGC Medium-Pressure Chromatography Systems offer a single preparative chromatography solution that scales to fit your application and throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

The columns used in FPLC are large [mm id] tubes that contain small [µ] particles or gel beads that are known as stationary phase. The chromatographic bed is composed by the gel beads inside the column and the sample is introduced into the injector and carried into the column by the flowing solvent. As a result of different components adhering to or diffusing through the gel, the sample mixture gets separated.

AVAILABLE COLUMNS

1.Bio scale^TM Mini UNOsphere^TM Q Catridge (Cat.#732-4102)

2.Bio scale^TM Mini Nuvia^TM IMAC Ni-Charged (Cat.#780-0811)

3.Bio scale^TM Mini UNOsphere^TM S Catridge (Cat.#732-4112)

4.Bio scale^TM Mini Ion Exchange Sampler Pack (Cat.#732-4650)